基于SPAD值的春石斛假鳞茎成熟度无损判定模型

温碧柔; 蔡雪妍; 杨逸; 彭杰良; 陈方尧; 闻真珍; 刘伟

doi:10.19303/j.issn.1008-0384.2022.06.013

基于SPAD值的春石斛假鳞茎成熟度无损判定模型

Noninvasive Determination on Maturity of Dendrobium nobile Pseudobulbs based on SPAD Measurements

摘要

摘要:
目的春化处理诱导开花效果不稳定是春石斛生产中经常出现的现象，其原因是气候差异导致的假鳞茎成熟的具体时间不一致，春化起始时间难于把握。为准确判断春石斛假鳞茎的成熟度，确定春化适宜的起始时间，建立了本模型。
方法以大龙虾（Dendrobium Hot Lobster）、Dendrobium Sakura Hime和甜心（Dendrobium Lucky Girls ‘Sweet heart’）3个春石斛品种为材料，测定不同成熟度春石斛假鳞茎自止叶向下的5个叶位叶片的叶绿素相对含量值（SPAD），通过该5个叶位SPAD值构建的函数关系建立了与假鳞茎成熟度的相关关系模型。以可爱河马（Dendrobium Lovly Hippo）及Dendrobium Sakura Hime 2个春石斛品种为材料，统计了经低温春化后假鳞茎的开花情况，以验证该模型的有效性。
结果 5个叶位叶片的叶的SPAD值之间的函数关系的k值（差异指数）与春石斛假鳞茎的成熟度相关，所构建的模型能够预测不同春化起始时间的春石斛开花情况，可用于春石斛适宜春化起始时间的判断。
结论本研究提出的基于叶位和SPAD值构建的春石斛假鳞茎成熟度无损判定模型，是一种无损、快速、准确的方法，可提高春石斛生产中盆花的品质，也为春石斛精准调控栽培技术的建立奠定基础。

Abstract:
Objective A mathematic model for accurate and noninvasive but oftentimes difficult determination on the maturation stages of orchid pseudobulbs was established for effective and timely vernalization of Dendrobium nobile.
Methods Chlorophyll SPAD measurements of 5 leaves from the stop leaf to the lower ones on a pseudobulb of varieties of D. nobile including Hot Lobster, Sakura Hime, and Lucky Girls Sweetheart orchids at different stages of maturity were obtained. A correlation between the measurements and pseudobulb maturity was established. Flowering of Lovely Hippo and Sakura Hime pseudobulbs after low temperature vernalization was monitored for model verification.
Results The k value (difference index) on the functional relationship between SPAD data and pseudobulb maturity indicated a viable predictability of the established model.
Conclusion The newly established method for determining the maturation stages of D. nobile pseudobulbs based on SPAD measurements of differently positioned leaves was noninvasive, rapid, and accurate. The application would significantly facilitate the operation of the orchid cultivation.

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