• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

红豆树异黄酮类成分抑制番茄灰霉病菌的活性研究

Inhibitory Activity of Isoflavones from Ormosia hosiei Seeds against Botrytis cinerea

  • 摘要:
      目的  研究红豆树提取物及其异黄酮对番茄灰霉病菌的抑菌作用,为植物源抑菌剂的开发和红豆树提取物利用奠定基础。
      方法  采用生长速率法筛选红豆树异黄酮对番茄灰霉病菌的抑制作用,进一步测定异黄酮鹰嘴豆芽素A对番茄灰霉病菌的菌丝干重、细胞膜、还原糖含量、过氧化氢酶、过氧化物酶和超氧化物歧化酶等保护酶活性的影响,探讨抑制植物病原真菌的生理生化的作用,评价单体在离体番茄上的抑菌效果。
      结果  鹰嘴豆芽素A对番茄灰霉病菌具有好的抑制效果,EC50值为203.189 μg·mL-1。番茄灰霉病菌经鹰嘴豆芽素A处理后,菌丝干重减少,细胞膜的通透性增加,菌体内还原糖含量减少,保护酶含量增多,并且在离体番茄上菌丝生长防治效果较好。
      结论  红豆树种子乙酸乙酯活性部位富含生物碱和黄酮类化学成分,其中异黄酮成分是关键的抑菌活性成分。异黄酮成分发挥抑菌活性的作用方式,可能与其干扰菌丝生长、使菌丝细胞膜正常功能受损以及降低菌丝体内保护酶活性等有关。

     

    Abstract:
      Objective  Antifungal activity of the isoflavones of Ormosia hosiei on Botrytis cinerea Pers was investigated for the development of a natural disease control agent on tomato plants.
      Methods  Inhibitory effect of the isoflavones extract from seeds of O. hosiei on the pathogen, B. cinerea, that infects tomato plants was tested according to the in vitro fungal growth rate. Functions of the isoflavone monomer biochanin A on the changes of mycelial dry weight, cell membrane, reducing sugar content, and activities of catalase, peroxidase, superoxide dismutase, and other protective enzymes of B. cinerea were analyzed. Physiological, biochemical, and bacteriostatic effects of the monomer on tomatoes were observed in vitro.
      Results  Biochanin A of O. hosiei significantly inhibited the growth of B. cinerea with an EC50 of 203.189 μg·mL−1. Its presence lowered the dry mycelia weight, cell membrane permeability, and reducing sugar content but rose the protective enzyme activities in hyphae of B. cinerea.
      Conclusions  The ethyl acetate extract from the seeds of O. hosiei was rich in alkaloids and flavonoids. Of which, isoflavones was believed to be the key active antibacterial components that inhibited the fungal growth by interfering the growth, impairing the cell membrane functions, and reducing the protective enzyme activities of the hyphae.

     

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