Objective   Expression and distribution of Mel 1a in various organs of Wanxi White Geese were studied to reveal the regulatory mechanism of melatonin in the animal.

  Method   Mel 1a mRNA and proteins from various tissues and organs of Wanxi White Goose were extracted for real-time PCR and western blot to determine the distribution and differential expressions.

  Result   Mel 1a protein was widely distributed in the brain, heart, kidney, liver, lung, spleen, pancreas, and muscle cells of the geese. The IOD analysis of immunohistochemically positive signals using Image-pro plus 6 showed that the spleen had the strongest signal, followed by the pancreas. The signal from the spleen was significantly stronger than the brain, and the signal from the lungs was significantly stronger than the heart and muscles.The expression levels of Mel 1 a mRNA and protein in various organs ranked in the order of ovary, pancreas, spleen, kidney, dry, heart, brain, lungs, and muscles. And they increased gradually along with the development of follicles and decreased after the follicle diameter exceeded 3 cm.

  Conclusion   Melatonin receptors exist in all tissues of western Anhui white geese, with the highest expression in the ovary. Melatonin can mediate the regulation of various biological functions through the receptor, especially the regulation of follicular development in western Anhui white geese.