多花黄精<i>ACY1</i>基因家族鉴定及其表达分析

张慧; 罗彬彬; 朱田圆; 班景洁; 张春渝; 李小利; 朱建明; 林玉玲; 赖钟雄

doi:10.19303/j.issn.1008-0384.2023.06.005

多花黄精ACY1基因家族鉴定及其表达分析

Identification and Functions of ACY1 Family in Polygonatum cyrtonema

摘要

摘要:
目的筛选鉴定多花黄精中氨基酰化酶（aminoacylase 1，ACY1）基因，为多花黄精抗逆基因功能研究奠定基础。
方法基于多花黄精转录组数据，对多花黄精氨基酰化酶（PcACY1）基因家族进行鉴定，分析基本理化性质、基因结构、保守基序、进化关系等，并采用qRT-PCR验证ACY1家族成员在不同组织器官中和盐胁迫处理下的表达模式。
结果多花黄精ACY1家族有2个成员，均为亲水蛋白，具有信号肽，无跨膜结构，亚细胞定位预测结果显示2个成员均定位于细胞质中。与植物其他物种ACY1构建进化树发现，其进化特性可分为2类。植物ACY1启动子顺式作用元件预测结果显示，ACY1存在多种激素、胁迫和生长发育相关的响应元件。qRT-PCR分析结果显示，PcACY1成员在多花黄精不同组织器官中的表达水平差异较大且受盐胁迫诱导。
结论 PcACY1在多花黄精生长发育和抵御逆境过程中可能发挥重要作用，这为进一步研究多花黄精ACY1的功能及遗传改良等奠定基础。

Abstract:
Objective Family of aminoacylase 1 genes (ACY1) in Polygonatum cyrtonema Hua was identified, and associated resistance functions analyzed.
Methods Based on the transcriptome data, the family of P. cyrtonema Hua aminoacylase genes (PcACY1) was identified. Physicochemical properties, structure, conserved motifs, and evolutionary relationships of the members were analyzed. Expressions of PcACY1 in tissues/organs and under salt stress were detected by qRT-PCR.
Results The PcACY1 family had two members located in the cytoplasm, both of which were hydrophilic proteins with signal peptides and no transmembrane structure. As shown in the phylogenetic tree, they exhibited two categories of evolutionary characteristics. The predicted cis-acting elements of the plant ACY1 promoter indicated the presence of multiple hormone-, stress-, and growth/development-related functions. PcACY1 displayed significantly differentiated expressions in different tissues and organs which could be induced by salt stress.
Conclusion PcACY1 appeared to play an important role in the growth and development as well as stress resistance of P. cyrtonema .

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