Abstract:
Objective Regulatory functions of Polygonatum cyrtonema Hua PLT (PcPLT) in the growth and development of rhizomes of the plant were investigated.
Methods Based on the P. cyrtonema transcriptome database, the identity and bioinformatics of PcPLT family were obtained. Relative expressions in tissues of the plant and that under NaCl stress were detected using the qRT-PCR technique. The fusion expression vectors of PcPLT2-2 and PcPLT2-7 were constructed, and their fluorescence signals examined to determine subcellular localization.
Results Fifteen PcPLT family members were identified. They were hydrophilic proteins, absent of intron structure, coded 159-601 amino acids, and evolutionarily closely related to Liliaceae Asparagus officinalis L. The predicted subcellular localization of PcPLT2-2 was in cytoplasm and nucleus, while PcPLT2-7 in nucleus only. PcPLT2-3 and PcPLT2-7 mostly in the rhizomes; and PcPLT1-3, PcPLT1-4, and PcPLT2-7 responsive to salt stress.
Conclusion PcPLT were tissue-specific and capable of enhancing the stress resistance of P. cyrtonema. They might act as an organ development regulator associated with the morphogenesis of rhizome expansion. If so, the result obtained in this study would be of value for the in-depth understanding of the biological functions of PLT.
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