Abstract:
Objective To improve the mycelium biomass of Lyophyllum decast liquid seeds, high-quality liquid seeds are prepared by optimizing the liquid fermentation formula, to promote the rapid development of industrial production of L. decastes mushrooms.
Methods Taking L. decastes as the research material, the effects of 6 kinds of carbon sources, 8 kinds of nitrogen sources, and 10 kinds of metal ions on the mycelium growth were analyzed by single-factor design. The carbon source, nitrogen source, and metal ions screened out by single-factor design were further optimized by the Box-Behnken response surface method, and the advantages and disadvantages of liquid seeds and solid seeds in the production of L. decastes mushrooms were compared.
Results The nutritional elements suitable for the growth of L. decastes were whole wheat flour, peanut meal, K2HPO4, and MgSO4·7H2O, and the optimal formula was whole wheat flour 47 g·L−1, peanut meal 22 g·L−1, K2HPO4 2.00 g·L−1, MgSO4·7H2O 2.00 g·L−1. When the fermentation culture reached the 8th day, the dry cell mass (DCM) was 32.81±1.10 g·L−1. Compared with the DCM by the initial formula, the DCM prepared with the optimized formula was increased by 7.34 times. Compared with solid seeds, the production time of liquid seeds was shortened by 48 days, the time of mycelium germination was shortened by 2 days, and the yield per bag increased by 15.47%, in industrial production.
Conclusion The liquid fermentation formula developed in this study can obtain high-quality production seeds, and the liquid fermentation strategy for the preparation of seeds is suitable for the industrial production of L. decastes mushrooms.
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