• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

紫糯特种稻紫两优737重要农艺性状遗传分析

Analysis of Genetypes Related to Important Agronomic Traits of Purple Waxy Special Rice Variety Ziliangyou 737

  • 摘要:
      目的  解析紫糯特种稻紫两优737及其亲本的产量、品质相关性状的基因型构成,为该品种及其双亲在育种和生产上的进一步应用提供分子依据。
      方法  应用10个重要基因的13个分子标记,分析紫两优737及其亲本紫392S、福恢737在产量、品质等相关基因座位上的等位基因变化。
      结果  在7个基因座位上,紫两优737及其亲本均携带相同等位基因型。在穗实粒数主效基因Gn1a座位上均携带高产等位基因Ha-Gn1a;在粒重和粒长主效基因GS3座位上均携带长粒型等位基因MH-GS3;在种皮基因Pb座位上均携带紫色果皮等位基因型;在糯稻蜡质基因wx座位上均携带低直链淀粉含量等位基因型;此外在淀粉分支酶基因SBE1SBE3以及极限糊精酶基因PUL基因座位上亦均携带相同等位基因型。在3个基因座位上,双亲携带不同等位基因型。在Hd3a座位上,紫392S、福恢737和紫两优737分别携带Hd3aNiphd3aKasaHd3aNip/hd3aKasa;在Hd1座位上,分别携带Hd1japHd1indHd1jap/Hd1ind在焦磷酸化酶大亚基基因AGPlar座位上,分别为Ⅰ型,Ⅱ型及Ⅰ/Ⅱ杂合型。
      结论  紫两优737重要农艺性状的遗传解析为其具有高产、优质、适应性好及种皮颜色等特点提供了分子证据,并为相关有利等位基因的进一步利用提供参考。

     

    Abstract:
      Objective  This study was conducted to analyze the genotypes of yield and quality-related traits of the purple waxy special rice Ziliangyou 737 and its parents, and to provide molecular basis for the further application of this variety and its parents in breeding and production.
      Method  Using 13 molecular markers of 10 important genes, the alleles of Ziliangyou 737 and its parents Ziliang392s and Fuhui 737 were analyzed in relation to yield and quality.
      Result  In 7 gene loci, both Zizliangyou 737 and its parents carry the same allele genotype. The high-yield allele Ha-Gn1a was carried in the seat of Gn1a, the major gene of panicles number. The long grain allele MH-GS3 was carried in the GS3 locus of the major gene for grain weight and grain length. The alleles of purple pericarp were carried in the Pb locus of seed coat gene. All alleles with low amylose content in waxy gene wx locus were carried in glutinous rice. In addition, the same allelic genotype was carried in the starch branch enzyme genes SBE1 and SBE3 and the limit dextrin enzyme gene PUL gene locus. In three loci, parents carry different alleles. In the Hd3a seat, the Zi 392S, Fuhui 737 and Ziliangyou 737 carry Hd3aNip, hd3aKasa and Hd3aNip/hd3aKasa respectively. On the Hd1 seat, carry Hd1jap, Hd1ind and Hd1jap/Hd1ind respectively. In the large subunit gene AGPlar locus of pyrophosphorylase, there were type I, type II and hybrid type I/II, respectively.
      Conclusion  The genetic analysis of important agronomic traits of Ziliangyou 737 provided molecular evidence for its high yield, high quality, good adaptability and seed coat color, and provided reference for further utilization of related favorable alleles

     

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