Abstract:
Objective This study was conducted to analyze the genotypes of yield and quality-related traits of the purple waxy special rice Ziliangyou 737 and its parents, and to provide molecular basis for the further application of this variety and its parents in breeding and production.
Method Using 13 molecular markers of 10 important genes, the alleles of Ziliangyou 737 and its parents Ziliang392s and Fuhui 737 were analyzed in relation to yield and quality.
Result In 7 gene loci, both Zizliangyou 737 and its parents carry the same allele genotype. The high-yield allele Ha-Gn1a was carried in the seat of Gn1a, the major gene of panicles number. The long grain allele MH-GS3 was carried in the GS3 locus of the major gene for grain weight and grain length. The alleles of purple pericarp were carried in the Pb locus of seed coat gene. All alleles with low amylose content in waxy gene wx locus were carried in glutinous rice. In addition, the same allelic genotype was carried in the starch branch enzyme genes SBE1 and SBE3 and the limit dextrin enzyme gene PUL gene locus. In three loci, parents carry different alleles. In the Hd3a seat, the Zi 392S, Fuhui 737 and Ziliangyou 737 carry Hd3aNip, hd3aKasa and Hd3aNip/hd3aKasa respectively. On the Hd1 seat, carry Hd1jap, Hd1ind and Hd1jap/Hd1ind respectively. In the large subunit gene AGPlar locus of pyrophosphorylase, there were type I, type II and hybrid type I/II, respectively.
Conclusion The genetic analysis of important agronomic traits of Ziliangyou 737 provided molecular evidence for its high yield, high quality, good adaptability and seed coat color, and provided reference for further utilization of related favorable alleles