• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

猫尾草叶绿体基因组结构及其解析

Structure of Uraria crinita Chloroplast Genome

  • 摘要:
      目的  阐明岭南药食两用植物猫尾草的叶绿体基因组结构特征,为猫尾草资源的保护、利用和开发提供理论依据。
      方法  采用高通量测序技术开展猫尾草叶绿体基因组测序,并通过生物信息方法进行拼接、注释、解析以及系统进化分析。
      结果  猫尾草叶绿体基因组是149 774 bp的环状双链四段式分子,包含128个基因,GC含量为38.2%。猫尾草叶绿体基因组含有26 015个密码子,偏好以A或T结尾;存在110个简单重复序列,以A或T单核苷酸重复居多。序列比对和进化分析显示猫尾草与同属狸尾豆的亲缘关系最近。
      结论  首次报道猫尾草叶绿体基因组的全序列,并明确其结构特点,为猫尾草的栽培育种、遗传多样性和资源利用等奠定基础。

     

    Abstract:
      Objective  Structure of the chloroplast genome from Uraria crinita, a plant used as food as well as herbal medicine in Lingnan area of China, was studied.
      Methods  The whole chloroplast genome was obtained by high-throughput sequencing and then assembled, annotated, and analyzed by bioinformatic means.
      Results  The genome was an annular quadripartite molecule of 149 774 bp that harbored 128 genes. It had a low GC content of 38.2% containing 26 015 codons that mostly ended with A or T. There were 110 loci of simple sequence repeat detected with the mononucleotide largely formed by A or T. An alignment and phylogenetic analysis on the sequence indicated a close relationship between U. crinita and U. lagopodioides.
      Conclusion  The chloroplast genome and structure of U. crinita were unveiled for the first time to aid future studies on the cultivation, breeding, genetics, and utilization of the valuable plant.

     

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