Abstract:
Objective Pathogen of the leaf spot disease on Acanthus ilicifolius was isolated and identified with its biological characteristics determined.
Method Microbes were isolated from the diseased A. ilicifolius leaves collected from the Zhangjiangkou Estuary Mangrove National Nature Reserve in Fujian Province by the detached tissue method. Morphological and molecular biological techniques including ITS and EF1-α gene sequence analysis were employed to identify the pathogen. Optimal growth conditions of the identified fungus with respect to temperatures, pH, carbon sources, and nitrogen sources, in addition to its activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), laccase, cellulase (CL), andβ-glucosidase (β-GC) were determined.
Result For the first time Fusarium verticillioides in the Ascomycota phylum was identified as the responsible pathogen of the disease. It thrived at 25°C and pH 10 on a medium using starch as carbon source and yeast powder as nitrogen source. POD was the oxidoreductase, and CL the hydrolase, that showed the highest activity in the fungus.
Conclusion The pathogen that caused the leaf spot disease on bougainvillea in the Zhangjiang Estuary was identified as F. verticillioides. The biological characteristics of the strongly adaptable fungus were determined.
下载: