• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

多粘类芽孢杆菌NPDY05-8对玉米茎基腐病的防治效果及对土壤微生物的影响

Control on Maize Stalk Rot and Effects on Soil Microbes of Paenibacillus polymyxa

    摘要
  • 摘要:
      目的  探明多粘类芽孢杆菌NPDY05-8灌施土壤后对玉米茎基腐病的防治效果，及该菌在土壤中定植情况和对土壤的细菌和真菌等微生物群落的影响。
      方法  利用Illumina MiSeq 高通量测序技术，以未灌施菌株NPDY05-8和种植花生轮作的土壤为对照，对灌施多粘类芽孢杆菌NPDY05-8试验地土壤的细菌及真菌群落组成结构进行研究分析。
      结果  该菌株对玉米茎基腐病的田间防治效果在2021年达90.92%，2022年达95.68%，显著优于化学药剂的防效。土壤菌群分析结果显示菌株NPDY05-8能够在土壤中定植，且长期存在于土壤当中。施用菌株NPDY05-8一年Y1和30日Y2的土壤细菌多样性OTUS分别为1 096个和1 149个，均高于未施用菌株的土壤Y3的973个及轮作土壤的941个；赤霉菌属的丰度Y1为4.15%、Y2为8.76%，均低于未施用菌株的土壤Y3的17.18%；镰刀菌属的丰度Y1为0.80%、Y2为1.12%，均低于未施用菌株的土壤Y3的2.17%；施用菌株一年的土壤与施用菌株30日的土壤在细菌和真菌水平的相关性指数高，距离最近。
      结论  灌施菌株NPDY05-8能改变土壤的真菌及细菌的种群结构，控制有害真菌，达到持久改良土壤和防治土传性真菌病害的目的。

     

    Abstract:
      Objective  Control on maize stalk rot and effects on soil microbial communities by the addition ofPaenibacillus polymyxa in soil were studied.
      Method  On a peanut rotational cultivation field, composition and structure of the microbial communities in the soil were determined using the illumina MiSeq high-throughput sequencing technique to compare with those on the land incorporated with P. polymyxa NPDY05-8.
      Result  The disease control on the maize stalk rot by addition of NPDY05-8 in soil reached 90.92% in 2021 and 96.58% in 2022 as shown by the experiment. Those were significantly more effective than what was achieved by using chemical treatments. NPDY05-8 continued to present in soil after the colonization in a year (Y1) with an OTU of 1 096 and in 30-d (Y2) of 1 149, which were significantly higher than control (Y3) of 941. Meanwhile, the population of Gibberella at 4.15% in Y1 and 8.76% in Y2 were significantly lower than that in Y3 at 17.18%; and that of Fusarium at 0.80% in Y1 and 1.12% in Y2, significantly lower than that in Y3 at 2.17%.
      Conclusion  Addition of NPDY05-8 in maize field altered the microbial community in the soil that significantly provided a lasting control effect on the pathogenic fungi for maize farming.

     

    • HTML全文
    • 参考文献(25)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回