• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

玉屏风煎剂对德化黑鸡生长性能、免疫调节、血清生化指标的影响

Effects of Yupingfeng Decoction on Growth Performance, Immune Function and Serum Biochemical Indices on Dehua Black Chicken

    摘要
  • 摘要:
      目的  探讨玉屏风煎剂对德化黑鸡生长性能、免疫调节、血清生化指标的影响，并对其安全性进行观察。
      方法  黄芪、白术、防风按质量比2:2:1混合煎制，浓缩成含生药1 g·mL−1的玉屏风煎剂。将30日龄体重相近的德化黑鸡160头随机分为低剂量组（1.25 mL·头−1·天−1）、中剂量组（1.875 mL·头−1·天−1）、高剂量组（2.5 mL·头−1·天−1）和空白对照组（0 mL·头−1·天−1），每组4个重复，每个重复10头，煎制玉屏风饮水给药，饲喂试验为期25 d；试验第25 d采血及十二指肠，检测生长性能、十二指肠形态结构、免疫功能、抗氧化能力、血清生化等相关指标。
      结果  与对照组相比，1）中、高剂量组日增重分别提高16.77%（P＜0.05 ）、27.58%（P＜0.01 ），3个剂量组饲料转化率分别提高8.30%、14.61%、21.92%；2）3个剂量组血清IL-2含量，中、高剂量组十二指肠黏膜SIgA水平显著提高（P＜0.05或P＜0.01）；3个剂量组血清 IFN-γ、TNF-α含量显著降低（P＜0.05或P＜0.01）。3）3个剂量组MDA浓度极显著降低（P＜0.01），GSH-Px、SOD活性极显著升高（P＜0.01），高剂量组T-AOC、CAT活性显著升高（P＜0.05或P＜0.01）。4）中、高剂量组隐窝深度显著降低（P＜0.05），绒隐比显著增加（P＜0.05或P＜0.01）；5）3个剂量组总胆红素浓度、甘油三酯浓度、总胆固醇浓度、谷草转氨酶活性、谷丙转氨酶活性、碱性磷酸酶活性、肌酐浓度、肌酸激酶活性差异均不显著（P＞0.05）。
      结论  玉屏风煎剂可以提高德化黑鸡的生长性能，改善肠道形态结构，强化抗氧化能力，增强免疫功能，具有抗炎、维持免疫平稳态的作用，使用安全，最适剂量为2.5 mL·头−1·天−1。

     

    Abstract:
      Objective  Effects of Yupingfeng on the growth performance, immune function and serum biochemical indices of Dehua black chicken were investigated, and the safety of Yupingfeng was observed.
      Method  Astragalus membranaceus, Atractylodis macrocephalae Koidz. and Radix Saposhnikoviae were mixed and decocted at a mass ratio of 2:2:1 to make the decoction concentration at 1 g·mL-1 of raw materials. The tested 160 30-day-old Dehua black chickens with similar body weight were randomly divided into a low-dose group (treated with 1.25 mL Yupingfeng decoction per chicken per day), a medium-dose group (treated with 1.875 mL), a high-dose group (treated with 2.5 mL), and a control group（0 mL). Each group had 4 replicates, with 10 chickens in each replicate. Yupingfeng decoction was mixed in drinking water and fed for 25 days. Blood and duodenum were sampled to test relevant indicators of growth performance, intestinal morphology immune function, antioxidant capacity and serum biochemistry.
      Results   Compared with the control group, 1）the daily weight gains in groups of the medium- and high-dose were increased by 16.77%(P＜0.05) and 27.58%(P＜0.01), and the feed conversion ratios of the three dose groups were increased by 8.30%,14.61% and 21.92%, respectively; 2）the levels of IL-2 of serum in the three dose groups, and the levels of SIgA of the duodenal mucosa in the medium- and high-dose groups were significantly increased(P＜0.05 or P＜0.01) ; the levels of IFN-γ and TNF-α of serum in the three dose groups were significantly decreased (P＜0.05 or P＜0.01) . 3) the reduction of the MDA concentration in the three dose groups was extremely significant (P＜0.01), while the GSH-Px activity and SOD activity in the three dose groups, the CAT activity and T-AOC activity in the high dose group, were significantly increased(P＜0.05 or P＜0.01). 4) In the medium- and high- dose groups, the crypt depth was decreased (P＜0.05), while the ratio of villus height to crypt depth was increased(P＜0.05 or P＜0.01); 5) In the three dose groups , there were no significant differences in the serum biochemical indices of total bilirubin, triglycerides, total cholesterol, aspartate aminotransferase alanine aminotransferase, alkaline phosphatase, creatinine, and creatine kinase (P＞0.05).
      Conclusion  The Yupingfeng decoction can improve the growth performance, intestinal morphology, antioxidant capacity and immune function with anti-inflammatory effect and maintaining immune homeostasis in Dehua black chicken. And Yupingfeng decotion is safe, and the proper dosage is 2.5 mL per chicken per day.

     

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