• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

四川地区甜樱桃S基因型及SFB4'基因的分子鉴定

Identification of S Genes and SFB4' in Cerasus avium L.

    摘要
  • 摘要:
      目的  甜樱桃（Cerasus avium L.）的S基因决定其自交的亲和性。为鉴定四川地区甜樱桃品种的S基因型和高自交亲和性品种，本研究对收集到的39份甜樱桃材料进行S基因型鉴定，并比较SFB4与其自交亲和突变体SFB4'基因的差异。
      方法  利用S基因通用引物和特异引物对四川地区种植的39份甜樱桃叶片DNA进行PCR扩增，并对含有SFB4/SFB4'基因的扩增片段进行测序。
      结果  39份甜樱桃材料共鉴定出S1、S2、S3、S4、S6、S9等6个基因型，其中27份材料含有S3基因，19份材料含有S9基因，12份材料含有S1基因，11份材料含有S4基因，7份材料含有S6基因，3份材料含有S2基因。根据鉴定得到的S基因型结果，扩增得到含有S4基因的11份材料中的SFB基因的ORF序列，比对结果显示，相比于SFB4，SFB4'在903 bp处存在4个碱基缺失；本研究设计了SFB4'特异引物，测序结果显示，有7份材料的SFB基因型为SFB4'。
      结论  共鉴定得到S1、S2、S3、S4、S6、S9等6个基因型，对含有S4基因的11份材料的S4基因全长进行测序，确定7份材料为突变基因SFB4'。本研究为四川地区甜樱桃授粉树配置提供了数据支撑，并为甜樱桃新品种的选育奠定了理论基础。

     

    Abstract:
      Objective   Varieties of sweet cherry cultivars from Sichuan Province were analyzed for self-compatibility by the presence of mutant S gene.
      Method   Using the universal and specific primers of S genotype, DNA from the leaves of 39 Cerasus avium L. specimens collected in Sichuan were amplified by PCR. The amplified fragments containing SFB4 and/or SFB4' were sequenced for comparison and further analysis.
      Result   Six genes of S genotype were identified from the 39 sweet cherry specimens with 27 of them having S3 genes, 19 having S9, 12 having S1, 11 having S4, 7 having S6, and 3 having S2. In the 11 specimens containing S4 genes, the ORF sequences of SFBs were amplified. By comparing SFB4 and SFB4', 4 base deletions at 903 bp were observed on the mutant. Subsequently, with a specially designed primer for SFB4', the sequencing indicated the SFB in 7 of the specimens to be SFB4'.
      Conclusion   Six S genes were identified in the 39 C. avium specimens collected in Sichuan. The S4 from 7 of the specimens was the mutant gene, SFB4'.

     

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