• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

黄鳍东方鲀线粒体基因组结构及系统发育研究

Mitochondrial Genome and Phylogeny of Takifugu xanthopterus

  • 摘要:
      目的  研究我国大洋河水域黄鳍东方鲀(Takifugu xanthopterus)的分子遗传学特性,为黄鳍东方鲀种质资源遗传多样性和系统发育分析提供基础数据。
      方法  利用高通量测序技术,获得黄鳍东方鲀线粒体基因组全序列,分析线粒体基因组结构。
      结果  黄鳍东方鲀线粒体基因组序列全长16444 bp(GenBank 登录号:MT560586),由13 个蛋白质编码基因、22 个 tRNAs 基因、2 个 rRNAs 基因、1个控制区和1个轻链复制起始区组成。13个蛋白质编码基因中仅有cox1基因起始密码子为特殊的GTG,其余蛋白质编码基因均以ATG为起始密码子,cox2nad4终止密码子以单独的T结尾。偏好密码子(RSCU>2)分别是Arg(CGA)和Ser(UCC)。22 个tRNAs基因除trnS1基因外,其余tRNAs二级结构均为典型的三叶草结构。以斑腰单孔鲀(Monotreta leiurus)为外类群,对16种东方鲀属线粒体基因组全序列进行进化关系分析。由贝叶斯法构建的系统发生树表明,黄鳍东方鲀与痣斑多纪鲀(T. chrysops)和豹纹多纪鲀(T. pardalis)的亲缘关系最近。
      结论  黄鳍东方鲀的线粒体基因组结构和基因排列顺序符合鱼类基因组典型结构和排列特征。

     

    Abstract:
      Objective   Mitochondrial genome of Yellowfin Puffer (Takifugu xanthopterus) was studied to analyze the genetic diversity and phylogeny of the fish.
      Method  Mitochondrial genome of T. xanthopterus found in Dayang River was sequenced by high-throughput sequencing technology.
      Result  The complete mitochondrial genome was 16444 bp (GenBank accession No. MT560586) consisting of 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs, one control region, and one OL. The 13 protein-coding genes started with ATG codon except for cox1, which started with a special GTG codon. Cox2 and nad4 ended with codon T. The preference codon (RSCU>2) included Arg (CGA) and Ser (UCC). All 22 tRNA genes, except trnS1, had the typical cloverleaf-like secondary structure. Taking Monotreta leiurus as the outgroup, the complete mitochondrial genome sequences of 16 species of pufferfish were analyzed for evolutionary relationship. The phylogenetic tree constructed by Bayes software showed that T. xanthopterus was closely related to T. chrysops and T. pardalis.
      Conclusion  The mitochondrial genome structure and gene order of T. xanthopterus as analyzed seemed to be typical of fish.

     

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