香蕉离体试管芽诱变育种的研究Ⅰ.试管芽的60Coγ射线辐照试验
Study on Mutation Breeding of Banana in Vitro Tube-budsⅠ.Experiment on 60Co Gamma Ray lradiation of Tube-buds
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摘要: 60Coγ射线对天宝香蕉试管芽的辐照试验结果表明:供试试管芽材料因大小不同对60Coγ射线所表现的敏感性有明显的差异。其中以2.0-5.0mml/2纵切芽体最敏感,其次是2.0-5.0mm芽,而5.0～10.0mm芽最不敏感,它们的半致死剂量(LD50)分别是49.299Gy、57.431Gy和68.191Gy.60Coγ射线对供试试管芽的生长量和芽增殖数有明显的影响,其严重度与剂量水平成负相关。它们的相关系数(r)分别为-0.879,-0.906.Abstract: The experimental result of the irradiation of 60Co gamma ray to "Tianbao Banana" showed that the sensitivities of tube-buds the diferent size to 60Co gamma ray were quite discrepant, of which the longitudinally dissected tube-buds of 2.0-5.0 mm were the most sensitive, the 2.0-5.0 mm buds were the next and the 5.0-10.0 mm buds had the laest sensitivity.Their semi-lethal doses were 49.299Gy, 57.431Gy and 68.191Gy respetivdy.The growth weight and proliferation quantity of experimental tube-buds after irradiation had obvious negative correlation to dose leves.Their rdative coefficents(r) were -0.879 and-0.906 respectively.
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Keywords:
- Banana /
- 60Co gamma ray irradiation /
- Mutation breeding
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[2] De Guzman.E.V.et al.Plant regeneration from unirradiated and irradiated banana shoot-tip tissue cultured in Vitro,Philinn.Aaxic.1980.63:140-146
[4] Novak,F,J.et al.Mutation induction by gamma irradiation of in vitro cultured shoot-tips of banana end plentaln(Musacvs.).Trop.Agric.(Trinidad)1990,67:21-28
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