激素和氮素对无籽西瓜组培中芽分化的效应

Effect of Auximone and Nitrogen on the Gemmiparous Growing in Tissue Culture for Seedless Watermelon

摘要: 探讨了不同激素对无籽西瓜组培中芽分化的效应。结果表明,6-BA对促进芽分化有极显着作用,适用浓度为0.5～2.0mg/1;IBA对芽的生长有显着影响,适用浓度为0.5～1.5mg/1;Ad(腺素)主要是促进芽苗的伸长,适用浓度为20～80mg/l.在总氮量不变的情况下,进行了不同与比值的试验NH4+与N03-,结果显示,这两种不同形态氮素的配合使用是必需的,适用比值为0.2～1。

Abstract: The experiments were made to determine the optimal concentrations of auxirnones(6-BA,IHA,Ad)and the best ratio of NH4+:N03-for the gemmiparous growing in the tissue culture.The results showed that 6-BA promoted the viviparous growing significantly with the concentration of 0.5～2.0mg/l,IBA affected the bud growth with that of 0.5～1.5mg/l,Ad related to the straight growth with that of 20～80mg/l.Both ammonia and nitrate were necessary with the optimal ratio of 0.2～1.