Abstract: Systemic investigations orr the pathogons of outbreaks of an infectious disease in softshell turtles were carried out. 63 pure cultutes of the pathogen isolates from liver, intestine,kidney, heart and blood of 23 morbiund turtles were obtained, 45 of the isolates were identified to belong to Aeromoseas hydrophila which were gram-negative,short rod with a single polar flagillum,motile, oxidase and catalase positive,glucose fermenting and resistant to ampicillin but sensitive to a number of other antibiotics tested. The A. hydrophila was confirmed as a majar pathogen which caused an outbreaks of infectious disease in soft-shell Turtle.