Abstract: There existed a large number of beneficial antagonistic bacteria against Phoma asparagi Sacc.in the root soil of asparagus crops,among which A06,A209,and S34,have been selected to inhibit P.asparagi.The girdle bands of antagonistic bacteria against the mycelium growth were 10.6,10.0,8.6mm respectively,that against the spore germination were 9.3,8.7,9.2 mm respectively.The fermentation liquid of the bacteria had the strong inhibition to the disease.The suitable conditions for the bacteria growing were 25-35℃ temperature with 6-8pH.The antagonistic bacteria were wounded and restrained under ultraviolet in the distance of 300-1000 mm in 5 minutes.The efficiency of controlling the disease using the product of the antagonistic bacteria in the field was about 44.6%.