甘蔗胚状体的诱导与再生植株农艺性状观察

Embryoid Inducement and Observation on Agronomic Characters of the Regenerated Plant of Sugarcane

摘要: 甘蔗组织培养中,在前人报道使用N6(或MS)+2,4-D2.2mg/L,从外植体上产生的愈伤组织直接诱导出胚状体的基础上,再加进水解酪蛋白1.5g/L,,胚状体的诱导率显著提高,再生植株的蔗茎工艺成熟期株高比蔗茎苗植株平均高12.49cm、茎径小、0.3cm、每公顷有效茎数多8475条,产量低11.58% 、茎锤度下降5.35%.还讨论了提高胚状体再生植株蔗茎含糖分的方法.

Abstract: It was reported that the application of N6(or MS)+2,4-D(2.2 mg/L)may directly induce somatic embryoid from explant callus.Based on this,we added hydrolytic casamino acid(1.5g/L)and signi-ficantly increased the embryoid-inducing rate. The average height of regenerated plants at technicalmaturing period was 12.49 cm higher than that of stem-buding plants,its stem diameter was 0.3 cm thiner,the number of millable stalk was 8475/ hm2 more,the yield was 11.58% lower and the stem brix was 5.35% lower than that of stem-buding plants. The methodology to improve the sugar-content of embryoid-regenerated plants was also discussed in this paper.