Abstract: Based on previous construction of electron genetic map for MingHui 86 DNA micro-satellite markers(SSR),24 high polymorphism markers were obtained in distribution on all 12 rice chromosomes.By the markers,polymorphism analysis for 139 rice germplasm was conducted and 142 alleles were obtained with the 24 loci distributed in range between 2 to 10 and average at 5.9.The identifying capacity of the markers is higher for hybrid combinations than for hybrid rice parents.Eighteen hybrid combinations were compared with their parents by marker-matching analysis.In accordance with the identification in high genetic reliability of maternal lines,two false hybrid were eliminated from the sample base.A molecular identity database was constructed based on the137 rice materials with?24 marker loci,which could be directly applied for parental identification and miscellaneous seeds.