Abstract: the inductions of rice embryo calli are inhibited by both Kanamycin(Km)and Ampicillin(Ap),two kinds of Antibiotics, moreover, Km is more effective than Ap, Callus induction rates are negatively and significantly correlated with Antibiotic concentrations, Antibiotics.especially Km, also inhibit the growth of calli in the induction phase, No any green seedling differentiates with Km treated either in induction phase or in differentiation phase,except that a few albinoseedlings appear in the treatments of 50 and 100 mg/kgm,Differentiations of green seedlings are promoted by50 to 200 mg/kg of Ap treated in differentiation phase.Callus death rates rise as the increases of Km concentrations treated in induction phase, Treatments withKm and Ap in differentiation phase tend to cause the death of calli in varying degrees,but death rates are lower, Km+Ap inhibit coordinately the inductions and differentiations of calli.Ap can partially remove the inhibition effects of Km on differentiation of green seedlings when treated in differentiation phase.Discussions also have been made on the proper concentrations of Km andApused to detect whether the foreign genes were introduced in.