Abstract: The experiments were conducted to investigate whether or not egg yolk antibodies can be used as a functional egg yolk powder preparation in the prevention and treatment for enteric infection caused by pathogenic Escherichia coli.The major results were as follows: 1) agglutination reaction appeared after the egg yolk antibodies mixed with the pathogenic Escherichia coli,and the bacteria activity decreased which's not agglutination;2) the mortality rate of mouse was 60% after challenge with 1.3109 CFUmL-1 Escherichia coli pathogen K88,K99 and 987P,while the mortality rate of mouse decreased to 10% after the pathogen mixed with egg yolk antibodies;3) after feeding functional egg yolk powder for 30 days and challenge with 2.6109 CFUmL-1,the mortality rate of mouse was 40% compared with the control group in 84%.Comparing with control group,the serum ALT,AST and ALP were significantly lower in treated mouse and the organ index of liver,spleen,thymus,the intestinal villus height and V/C ratio were significantly higher.The results indicated that the functional egg yolk powder can be prepared to prevent and treat the infection disease caused by pathogenic E.coli and effectively to improve the growth performance of mouse.