Abstract: Agaricus biazei Murvill has been artificially cultured in Japan and Taiwan of China, but the yield was low.In order to further find out its characters, increase the yield and improve the culture technique, some characters of Agaricus btazei Murvill have been explored.The investigation results showed that,a).its spores are ellipse-shaped 6.5×5.0μm:locd-shaped association was found in growing mycellium, the width is arround 5～6μm, b).the optical termperature for the growth of mycellium is 22～23℃, suitable pH is 6.5～7.5, optical humidity in culture medium is growing material:water=1:2.4.optical carbon source is glucose and nitrogen source is(NH4)2SO4, corn meal and wheat bran.c).the optiacl temperature for the development of fruit body is 18～21℃, the air relative humidity is 85～95%.d).adding 864 bacterial solution into culture medium during femetation process may enhance the growth of mycellim, development of fruit body and yield increased 54%to compare with check treatment.e).polysaccharide content in fruit body is 6.55%, protein content is 28.70%, the total aminoacid in each l00 g protein is l9.22 mg,among which the human necessary aminoacids are 9.10 mg and occupied 47.33%of total content rough fat is 2.881%.Agaricus biazei Murvill is a valuable edible fungi.