福建芦笋茎枯病初侵染源和病菌特性的研究
Studies on Initial Infection Source and Physiological Character of Phoma asparagi Sacc.In Fujian
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摘要: 本文对芦笋茎枯病初侵染源和病菌特性的研究作了阐述。结果表明,病害初侵染源主要来自残留田间病茎和病株。病菌置放田地300d和埋土深15cm30d,病茎上病菌均能存活。成熟器孢子在PSA培养基上分别置5~25℃测定萌发率,经24h均能萌发,经48h仅10℃和15℃两处理有萌发力,经72h均丧失萌发力。分生孢子器形成最适相对湿度95%~100%,温度20~30℃,PSA培养基上菌丝体生长最适温度20~30℃,碳源为甘油,pH7~8。Abstract: This paper dealed with the initial infection source and physiological character of Phoma asparagi Sacc.The investigation showed that the main sources of initial infection come from the diseased stem residues and plants of asparagus which overwintered in field.The pathogen could survive in the diseased stems on soil surface and 15 cm underground for 300 and 30 days respectively.The germination rate of pycnidiospores was determined under 5-25℃ on PSA medium.It germinated in all temperature treatments after 24 hrs, only germinated in 10℃ and 15℃ treatments after 48 hrs, and all failed to germinate after 72 hrs.The optimum relative moisture and temperature for producing pycnidia were 95%-100%and 20-30℃.respectively.on PSA medium, the optimum temperature,pH and carbon source for mycelial growth were 20-30℃, pH 7-8 and glycerin respectively.