无血(清)培养基MA12增菌剂的研制及应用Ⅰ.MA12无血(清)培养基增菌剂对禽多杀性巴氏杆菌增菌效果试验
Preparation of Serum-free Medium by MA12 Bacterial Synergist and its Application I.Effect of MA12 Bacterial Synergist on Increasing the Amount of Pasteurella multacida of Fowl Cholera in Sorum-free Medium
-
摘要: 从多种成分的组合配方中研制成一种增菌效果显著的MA12促细菌生长因子,在禽多杀性巴氏杆菌的培养基中,加入0.05—0.15%MA12可替代3—5%的血(清)浓渡,二者繁殖菌数相似。以此制备的禽霍乱荚膜亚单位苗对家禽具有良好的免疫原性。MA12是一种含植物生长素、微量元素等物质的增菌剂,可高压灭菌,使用方便,是一种廉价的血清代用品。本研究系国内首次报道。Abstract: One kind of growth-stimulating factor, 12 which has an obvious bacterium-increasing effect had been prepared.Adding 0.05-0.15% MA12 to the culture growing Pasteurella multacida of Fowl Cholera has similar effect on increasing bacterial amount to compare with adding 3-5% animal serum in the culture.The cell capsule antigen prepared with serum-free culture has good immunity for poultry.MA12 is mainly consisted of plant growth-regulating substrate and microelements.It is easy to be gathered with low cost and convinient use,also can be sterilized by autoclave.This is the first report on using scrum-free medium to produce capsule subunit vaccine in China.
下载: