Abstract: The effects of factors,such as substratum,hardening of natural lighting,seedling age,temperature and root in vivo (twing cutting) on the first transitional transplant in factory growing seedling of loquat were studied,and the effect of second trasitional cultivation on the survival rate in soil culture was discussed.The results show that:1.The suitable substrata for trasitional cultivation of tissue culture seedling (TCS) are peat soil and the materials consisted of 70%peat soil and 30%black soil.2.The relationship between hardening of natural lighting from 0-30 days and the survival rate of first transitional transplant is rectilinear relation.The TCS should be hardened by natural lighting for 30 days before first transitional transplant.3.The survival rate of first transitional transplant is high in aged seedling.The seedling age of 40-60 days is suitable for transplant to reduce the growing seedling period.4.The average air temperature during first transitional transplant of TCS is about 20℃,and the suitable range is 15-24℃.The TCS can be transplanted all the year round,but in consideration of advantages and disadvantages the transplant of TCS would be kept away from summer.5.The relationship of survival rate of first transitional transplant between the taken root in vitro and that in vivo is shown no difference.Judged by the reduction of production costs and the increase in benefit,the taken root in vivo is better than that in vitro.6.The product between the ultimate survival rate of second transitional cultivation of TCS and that of soil culture is remarkable higher than the survival rate of TCS which directly planted in soil after first transitional transplant.Thus,in the growing seedlings factory of loquat,the TCS from first transitional transplant would be planted in soil after second cultivation (two mounths).Finally,the possibility of TCS transition without damnification by open culture was discussed.