红萍共生藻的体外培养

唐龙飞; 刘中柱; 渡边岩

红萍共生藻的体外培养

1.
福建省农业科学院红萍研究中心, 福州350013;
2.
菲律宾国际水稻所土壤微生物系, 马尼拉1099

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出版历程
- 收稿日期: 1990-12-14
- 刊出日期: 1991-11-14

Multiplication of Anabaena Azollae in Vitro

1.
National Azolla Research Center, Fujian Academy of Agricultural Sciences, Fuzhou, 350013;
2.
International Rice Research Institute, Manila 1099

摘要

摘要: 本研究首次采用红萍体内共生藻单克隆抗体为监测手段,直接从红萍共生腔内分离鱼腥藻进行培养.离体藻在无氮BG-11或者Allen培养基上迅速褪绿失活,在培养基中添加果糖、酵母提取物、氨基酸等可使其保绿期延长4倍左右.低氧分压(1% O2,99% N2)可进一步提高离体鱼腥藻保绿期,并略有增殖.单克隆抗体测定表明这些离体培养的红萍共生藻始终保持其固有的抗原性.
- 红萍共生藻 /
- 单克隆抗体 /
- 体外培养 /
- 少量增殖
Abstract: This experiment tried to isolate Andbaena azollae directly from Azolla leaf cavities after surface sterilization, then grew it on various culture media and conditions.More longer green-keeping periord of isolated Anabdena azollae was found in alien medium suplemented with organic N and C, micro-aerobic(1% O2, 99% N2)condition to compare with N-free medium and aerobic condition.The isolates underwent a limited multiplication in above growing condition but failed to continue the further multiplication.These cyanobacteria were antigenically identical to the endosybionts of Azolla.
- Anabaena azollae /
- Monoclonal antibody /
- artificial cultivation /
- Limited mutiplication

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参考文献(2)

[9] Bai K. Z., S. L. Yu, W. L. Chen and S. Y. Yang. 1978. Cultivation of alga-free A. wlla, isolation of Anabaena azollae and preliminary attempt at their association. In Proc. Symposium on Plant 'Tissue Culture. Science Press. Beijing. pp 455-458

[11] Franche C., and G. Cohen-Bazite. 1985. The structural nif genes of four symbiotic Anabaena awllae show highly conserved physical arrangement. Plant Sci., 39: 125-131

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文章访问数: 2494
HTML全文浏览量: 148
PDF下载量: 509
被引次数: 0

红萍共生藻的体外培养

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出版历程

Multiplication of Anabaena Azollae in Vitro

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出版历程

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