Abstract: This article deals with a promising effect on differentiation of the shoot apex of Trifoliate orange seedling and grafted citrange where it could indnce numerous clumped buds and develop into an plant with root, stem and leaf when it was cultivated in a MS in addition to Kt 2.0 mg/1, NAA0.1mg/l, Peptone 300mg/l and GA30.2mg/l, A test of Kt concentrations shows that Kt could inhibit the apical dominance and promote the germination of the axillary bud. The optimum concentration of Kt for the apex proliferation is 2.0 mg/l. An appropriate amount of peptone could help an apex inducing shoot and eventually form seedlings. A treatment of the base of a young shoot by soaking in 100 ppm 1 BA solution for se veral hours is helpful to root formation in vitro. Treated with 200 ppm ABT or 100 ppm IBA, a rootless shoot in vitro could successfully induce roots outside the tube, thus the propagation in vitro could be simplified considerably.