Abstract: The recombination were achieved by directly putting anabaena from megasporocarp of same or different Azolla species into the ma-tureor germinating megasporocarp which had been eliminated anabaena cells and the recombination rate is 10%. The recombined Azolla was firstly examined under microscope and tested the nitrogenase activity analysis as showing that Anabaena azollae existed in recombined Azolla frond which developed from megasorocarp previously eliminated anabaena cells.Secondly, the analysis by monoclonal antibody also proved the success in recombination and confirmed the newreestablished parnters are: Anabaena-free A. filiculoides譇nabaena azollae from A.microphylla; Anabaena-free A. filiculoides譇nabaena azollae from A. mexiconaj and Anabaeza-free A.microphyllaes譇nabaena azollae from A.Filiculoides and Anabaeza-free A. filiculoides譇nabaena azollae from A filicujo-idesj Anabaena-free A.microphylla譇nabaena azollae from A. microhylla respectively.