Abstract: Genomic DNAs of 41 wild Agaricus bisporus strains identified by esterase isozymes analysis,along with 8 cultivation strains and 14 foreign wild strains as controls,were extracted for SRAP and ISSR analyses.Internal transcribed spacer(ITS) sequences of partial strains were amplified and the sequences were aligned.The results showed that the homology of ITS sequences from the wild A.bisporus and the control strains surpassed 99%.Thus,ITS sequence could be used as DNA-level evidences for further identification.In addition,the results were consistent with those obtained by esterase isozyme identification.Cluster analysis showed that these wild strains could be divided into three major groups,which were geographically correlated,at the similarity coefficient value of 0.93.They were genetically distant from the control strains.It suggested that China had abundant germplasm resources of A.bisporus with unique genetic makeup.