Abstract: In laboratory,the soil respiration,dissolved organic carbon(DOC) and microbial biomass carbon(MBC) at 10℃,20℃ and 30℃ with different fertilizations in soil from a longan orchard were studied.The results showed that,in 85 days of incubation,(a)the CO2 production rate was high in the early stage followed by a rapid decrease and stabilization afterwards.The total cumulative amounts of CO2 ranged from 1439 to 4443 mg·kg-1;(b)temperature significantly contributed to the soil respiration-the soil temperature coefficient,Q10 was between 1.49 and 2.08,with the CK the lowest and the N5 treated the greatest;and(c)fungal waste application significantly increased soil respiration,as well as the soil DOC and MBC contents,while other fertilization treatments showed no significant difference.