Abstract:
Objective Pathogen of a newly emerging parasitic disease on Polygonatum cyrtonema Hua plants in Fujian was identified.
Methods A field investigation was conducted in P. cyrtonema production areas of Guangze County, Nanping City, Fujian to collect root and rhizosphere soil samples for isolating and identifying the parasitic nematode. Based on the morphological characteristics and molecular biological analysis, the isolates were screened and identified the caustic nematode with confirmation by an indoor infectivity test.
Results In the field, the nematode-infected P. cyrtonema plants grew slowly aboveground with stunted stature and yellowing chlorosis leaves. The parasite was morphologically and molecular biologically identified to be Rotylenchulus reniformis. In the soil of severe infestation, the nematode population could be as dense as 2,300 nematodes per 100mL. Mature females as well as gelatinous egg masses were observed to emerge from the roots of the plants grown indoor in soil inoculated with R. reniformis for 60d, indicating completion of a life cycle of the parasites.
Conclusion For the first time, the occurrence and causation of reniform nematode disease on P. cyrtonema Hua in the province were positively identified.
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