甘薯丛枝病去除病原技术的研究

A STUDY OF THE PATHOGENE-FREE TECHNIQUE ON SWEET-POTATO WITCHES’ BROOM

摘要: 应用抽气灭菌,两步培养法,以生根物质促进芽生长的三项技术,成功地从甘薯“新种花”丛枝病植株的茎尖培养中,获得了7%去除类菌质体的健康植株。应用形态观察、嫁接鉴定、氨基酸分析,证明其恢复到一般健康植株的外部形态与内部代谢应有水平。经3年8个月的室内培养和室外种植结果表明,其生长情况和生产性能良好,直接说明了上述三种方法相结合的验证技术是有效的。

Abstract: During past four years we have done a lot of work on sweet-potato shoot tip culture to eliminate witches’ broom pathogen and finally these plantlets have been observed carefully about their shape-appearances and aminoacid contents, and have beeii tested by graft to compare with those diseased plants, Result showed that 7% of these plantlets were assured of mycoplasmalike organism-free.We are sure this method is worth while in production of sweet-potato. of course, before planting these seedlings the insect vectors-leafhoppers in the field must be cleaned off thoroughly.

[6] 鲁雪华、陈扬春,1985, 通过茎尖培养获得去除类菌质体的甘薯植株及其验证技术的研究。作物学报 11(3):191-197

[7] 寥嘉信、钟美丽,1979,甘薯无病毒苗培育及病毒检定。中华农业研究 28(3):139-144

[11] Murashige,T. and F. Skoog.1962, A revised medium for rapid growth and bio-assays with tobacco tissue Culture. Physiol. plant 15:473-497