Abstract: Flavonoids are one kind of the most important substance in citrus fruits. Extraction methods for flavonoids (solvent method, microwave method, enzyme decomposition method, supercritical fluid extraction method and ultrasonic extraction method) were summarized, as well as isolation-purification method for flavonoids (column chromatography method, thin layer chromatography method, macroporous resin adsorption method and high speed counter current chromatography method,) All these provided reference for the study of flavonoids from Citrus. These data may supply useful information to extraction and purification of flavonoids from the citrus fruits.