Abstract: Resistant varieties Dular, which was known contains a resistance QTL qBlsr-11-1, and H359R, which was known contains three resistance QTL(quantitative trait loci) qBlsr3d, qBlsr5a and qBlsr5b, were used as resistance to bacterial leaf blight donor parents. On the basis of screening SSR markers closely linked to resistant QTL, we pyramided resistant QTL by bulk method combined with MAS. The results indicated that distances between the SSR markers and the target QTL estimated at 0.4-4.9cM, which will meet the basic requirements for MAS. MAS result indicated that pyramid 2-4 resistant QTL is an effective way to breed a new rice variety in Broad-spectrum resistant level.