Abstract: The major factors were analyzed and compared affecting the transformation frequency of indica rice mediated by Agrobacterium including medium, culture condition, subculture frequency, bacterial concentration and infection time and so on in the paper. At last a high transformation frequency of indica rice was set up. The results indicated that NMB medium for callus inducing and NB medium for callus subculturing were the best medium combination for tissue culture of indica rice. In the meanwhile, bacterial concentration, infection time and concentration ratio of KT and NAA in differential medium at the best level were modulated. The transformed calli were pre-differentiation. So the differentiation rate was improved significantly. The albino seedlings were reduced. Finally the transformation rate of indica rice was improved to 8%.