Abstract: Sixty one genotypes of cauliflower germplasm were analyzed for genetic diversity based on the morphological markers in this study. The results showed that there are obvious differences among 15 morphological characters in different germplasms, the average coefficient of variation was 68.10%, and it declared that they had a great potential variation. The first five principal components of the contribution rate was 73.21%, it indicated that the first five principal components contained all morphological markers to 73.21% of the content, so the first five principal components of cauliflower morphological traits were selected, 61 varieties could be divided into four groups according to threshold of 0.77. The results showed that clustering was able to separate the materials of coming from different areas and different maturing in a certain extent.