Abstract: The plantlets of Fujian wild grapes (Vitis amurensis Rupr.) were established by using its stems with node as explants. And its callus induction, subculture and long-term maintenance were carried out by using the stems and leaves of these plantlets. The results showed that, with the illumination of lights, the effect of medium MS+BA 2.0 mg稬-1+NAA 0.2 mg稬-1+ Sucrose 30 g稬-1 revealed the best on inducing of calluses for a rate of 100%. The medium MS+2,4-D 1.0 mg稬-1+ Sucrose 30 g稬-1 was the best for the callus subculture multiplication to obtain the friable calluses in good growth. For a long-term maintenance, the calluses were sub-cultured for several generations with the medium MS+2,4-D 1.0 mg稬-1+ Sucrose 30 g稬-1 and then followed with the sub-culture of medium MS+2,4-D 1.0 mg稬-1+KT 0.5 mg稬-1+AgNO3 5.0 mg稬-1+Sucrose 30 g稬-1 for one generation. Continually, the calluses were alternately sub-cultured with the two mediums and could grow vigorously with the illumination of lights.