Abstract: A new extraction technology-ultrasonic cycling extraction was applied to extract total antioxidant substances from Dictyophora indusiata. The optimal condition was investigated by six single factor experiments, with DPPH radical scavenging ability as antioxidant activity index. The best technical parameters were as follows:the ultrasonic extracting temperature was 40℃,the ultrasonic power was 600W, the intermittent ratio was 3:1(working 3sec and intermitting 1sec), the material to liquid ratio was 8% (w/v),the extracting time was 15min,the ethanol concentration was 80%.