发酵鱼粉中寡肽的测定

详细信息

Detection of Oligopeptides in Fermented Fishmeal

Fujian Precision Instruments Focus on Agricultural Testing Laboratory,Fuzhou,Fujian 350003,China

摘要: 采用16%单宁酸作为2000Da以上的高分子蛋白的沉淀剂,利用自动定氮仪和氨基酸自动分析仪分别检测滤液中蛋白质和游离氨基酸的含量,并计算出寡肽的含量。结果显示,测得1500Da的低分子量蛋白的回收率为90%~95%,相对标准偏差RSD为1.6%。
- 发酵鱼粉 /
- 蛋白质 /
- 游离氨基酸 /
- 寡肽 /
- 单宁 /
- 仪器测定
Abstract: 16% tannic acid was selected as precipitation agent for the polymer protein over 2000Da in Fermented fishmeal. The contents of proteins and free amino acids in the filtrate of fermented fishmeal were determined by the fully automatic azotometer and automatic amino acid analyzer, and then calculated the contents of oligopeptides. The results showed that the recovery rates of low molecular weight proteins of 1500Da were 90%~95% and the relative standard deviation was 1.6%.
- Fermented fishmeal /
- free amino acids /
- Oligopeptides /
- Tannin /
- determination

冯秀燕,计成.高效液相色谱法进行寡肽分离测定的研究[J].中国饲料,2001,20:25-27.