Abstract: This paper was aimed to investigate effects of different vernalization time on the growth of broad bean.With "Dapengyicun" bean as material, protective enzymes, MDA content and chlorophyll content of horsebean seedlings were tested at 0, 7, 14, 21, 28 days treated by vernalization. The results showed that as the increasing of vernalization time, activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) all increased firstly and then decreased.SOD and CAT activities peaked at 14 days, while the POD activity was at 21 days; The Malondialdehyde (MDA) content rose slowly at the early stage, then increased substantially at 28 days. No significant difference was detected on chlorophyll content as vernalization time extending.The result showed that vernalization time for bean seedling was the best when controlled around 14 days, not more than 21 days.