Abstract: Bacillus has very good biological characteristics due to its stability and high resistance, and then has been widely applied in industry, agriculture and medicine. Collection, preservation, appraisal system and the database of secondary metabolites of Bacillus strains were constructed. The Appraisal System contains the information of fatty acid, rRNA and physiological and biochemical property of Bacillus stains. The data of Secondary Metabolites were obtained from the GC-MS and LC-MS measurements. Then, a screening and evaluation system of microbial pesticides candidate strains will be formed, which can accelerate the screening of Bacillus strains. And then, researching on the strains which may have good potential application will be carried out rapidly and get ready for the industrialization.