Abstract: Diseased eels collected between 2006-2012 were classified by pidemiological, clinical symptoms and pathological characteristics, and 36 samples suspected to be infected by virus were identified. Total DNA was extraced from liver, spleen or kidney, and used for herpesvirus anguillae (HVA) detection. The results showed that herpesvirus anguillae were detected from 6 samples, and the disease of "Skin Mucous Debonding", "Septicemia", "Red Head", "Gill Rotten", which causing huge losses to the eel aquaculture industry, might caused by HVA; the amplified DNA sequence were exactely the same with the sequence from the starin of Taiwan, Japan and Europe, which means they might be the identical strain.