硫琼脂提取工艺的响应面法优化

刘秋凤; 苏永昌; 吴成业

硫琼脂提取工艺的响应面法优化

1.
福建农林大学食品科学学院, 福建福州 350002;
2.
福建省水产研究所, 福建厦门 361013

基金项目:

国家海洋公益性行业科研专项(201205022-4)

详细信息

通讯作者:
吴成业(1953-),男,研究员,研究方向:水产品保鲜、加工、综合利用及食品安全(E-mail:wcy@fjscs.ac.cn)

中图分类号: TQ461
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出版历程
- 收稿日期: 2012-06-24
- 修回日期: 2012-07-09
- 刊出日期: 2012-09-29

Optimization of Agaropectin Extraction with Response Surface Methodology

1.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2.
Fishries Research Institute of Fujian, Xiamen, Fujian 361013, China

摘要

摘要: 采用EDTA-Na2法提取硫琼脂,采用硫酸钡比浊法及苯酚-硫酸法分别测定硫琼脂的硫酸根含量和总糖含量。在单因素试验的基础上,利用响应曲面法优化提取工艺,建立了EDTA-Na2加入量(A)、洗脱时间(B)和洗脱温度(C)与硫琼脂的主要理化指标(硫酸根+总糖)含量(Y)之间的数学模型,确定了硫琼脂提取的最佳工艺条件为:EDTA-Na2加入量为5.90 g·L-1、洗脱时间2.2 h、洗脱温度为53℃。按此最佳工艺提取的硫琼脂的主要理化指标(硫酸根+总糖)含量为(84.00±0.45)%。
- 硫琼脂 /
- 提取工艺 /
- 响应面法
Abstract: Agaropectin was extracted by EDTA-Na2, the content of sulfate group and total sugar were determined by barium sulfate turbidimetry and hydrochloric acid and phenol-sulfuric acid method, respectively. Based on single factor experiments, the extraction technique for agaropectin was optimized using response surface methodology, the mathematical regression model was established for predicting the value of sulfate group content plus total sugar for agaropectin (Y) at different levels of adding EDTA-Na2(A), treating time (B) and temperature (C). The optimal extraction parameters for agaropectin were determined as: adding EDTA-Na2 5.90 g·L-1, treating time 2.2 h at temperature of 53℃, respectively. With the optimal parameters, the content of sulfate group plus total sugar for agaropectin extracted was 84.00±0.04%, which was in good agreement with the predicted value(Y).
- agaropectin /
- extraction technology /
- response surface methodology