Identification and Sequence Analysis of Tembusu Virus Isolated from White-feather Mule Ducks
-
摘要: 自肝脏表现不规则出血的白羽半番肉鸭脏器中分离获得1株病毒(命名为ZZ40株),经(RT-)PCR检测为鸭坦布苏病毒,可被鸭坦布苏病毒高免阳性血清特异性中和。应用鸭坦布苏病毒特异性引物从该株病毒基因组中成功获得结构蛋白基因片段,该片段与近年来我国分离的禽源坦布苏病毒株核酸序列同源性均在99.1%以上,而与蚊媒源坦布苏病毒MM-1775株同源性仅为88.6%;分子进化分析表明该株病毒与BYD-1等近年来的鸭坦布苏病毒分离株共处于一个进化分支。以上结果表明,我国白羽半番肉鸭存在坦布苏病毒感染,且该分离毒株的抗原性与我国早期分离于种(蛋)鸭的坦布苏病毒差异不明显。Abstract: A viral isolate (named as ZZ40) was abtained from liver of white-feather mule ducks with irregular hemorrhagic hepatitis, and were identified as duck tembus virus (DTMUV) by RT-PCR. The virus was specifically neutralized by anti-DTMUV hyperimmune serum. Gene fregments were abtained by using three sets of primers aimed at viral structural genes, and shared no less than 99.1% nucleotide sequence identity with those of avian tembus viruses isolated recently, and no more than 88.6% with that of Mosquito-borne tembus virus MM-1775 strain. Phylogenetic analysis showed that ZZ40 strain clustal into the same clade with those duck viruses isolated recently. These results indicated that the white-feather mule ducks in this case was infected with duck tembusu virus, which has no abviously variation in antigenicity compared with those of the early isolates of duck tembusu virus from egg-laying duck.
-
-
CAO Z, ZHANG C, LIU Y, et al. Tembusu virus in ducks, China[J].Emerg Infect Dis, 2011, 17(10): 1873-1875.
曹贞贞, 张存, 黄瑜, 等. 鸭出血性卵巢炎的初步研究[J]. 中国兽医杂志, 2010, 46(12):3-6. 傅光华, 黄瑜, 施少华, 等. 鸡黄病毒的分离与初步鉴定[J]. 福建畜牧兽医, 2011, (3):1-2. PLUMMER P J, ALEFANTIS T, KAPLAN S, et al. Detection of duck enteritis virus by polymerase chain reaction[J]. Avian Disease, 1998, 42(3): 554-564.
GBT 16550-2008, 新城疫诊断技术[S]. 2008. -
期刊类型引用(4)
1. 王强，孟巧巧，王煜，马丹炜. 两种桉树叶挥发物对蚕豆根尖细胞有丝分裂行为的影响. 四川师范大学学报(自然科学版). 2023(05): 685-692 . 
百度学术
2. 艾明君，尚禹函，赵婉钰，宋凯，刘婷钰，唐文静，莫金钢. 入侵植物三裂叶豚草化感作用的研究. 福建农业学报. 2022(10): 1326-1334 . 本站查看
3. 白雨濛，关彦俊，童晶晶，沙玉梅，刘雪梅，武小晖，宋凯. 蚕豆根尖微核技术. 吉林农业. 2019(04): 47 . 百度学术
4. 赵欢欢，付建涛，毛玉玲，黄卫娟. 蔗田污染物过硫酸铵和甲胺磷的快速精准检测方法探讨. 甘蔗糖业. 2019(06): 14-18 . 百度学术
其他类型引用(2)
计量
- 文章访问数: 2233
- HTML全文浏览量: 105
- PDF下载量: 372
- 被引次数: 6
下载:
