Genetic Variations of ORF5,NSP2 of Variant PRRSV-FJ09A strain and It's Pathogenicity
-
摘要: 采用RT-PCR方法及动物回归试验,对PRRSV-FJ09A毒株的ORF5与NSP2基因组进行遗传变异分析和致病性研究。结果表明:(1)与PRRSV-VR2332标准毒株比较,ORF5基因片段的核苷酸及推导的氨基酸的同源性分别为87.6%和88.1%,PRRSV-FJ09A毒株存在多个氨基酸发生突变,主要集中在抗原表位、毒力相关位点和糖基化位点;NSP2基因片段的核苷酸及推导的氨基酸的同源性分别为80.9%和77.0%,PRRSV-FJ09A毒株分3处共缺失31个氨基酸。(2)与国内2006年后流行的变异型PRRSV毒株比较, PRRSV-FJ09A毒株ORF5基因片段的核苷酸及推导的氨基酸的同源性分别为98.5%~99.7%和97.5%~99.5%, NSP2基因片段的核苷酸及推导的氨基酸的同源性分别为98.4%~99.3%和97.3%~98.9%,PRRSV-FJ09A毒株在NSP2基因片段多一处缺1个苯丙氨酸(F)。结论:PRRSV-FJ09A毒株为美洲型毒株,与国内流行的变异型PRRSV-FJ06A毒株的亲缘关系比较近。该毒株对30日龄的仔猪具有高致病性。
-
关键词:
- 变异型繁殖与呼吸综合征 /
- ORF5基因 /
- NSP2基因 /
- 遗传变异 /
- 致病性
Abstract: To fully understand the knowledge of variant porcine reproductive and respiratory syndrome virus (PRRSV) genetic variation and pathogenicity, the ORF5 and NSP2 genomes of PRRSV-FJ09A strain were amplified by RT-PCR and sequenced. Comparing with PRRSV-VR2332, ORF5 gene shared 87.6%of nucleotide homologies and 88.1% of the deduced amino acid homologies, there were point mutations within deduced amino acids, focus on amino acids of associated with virulence, epitope and N-glycosylation sites;NSP2 gene shared 80.9% of nucleotide homologies and 77.0% of the deduced amino acid homologies, and it shared a common feature of 31 amino acids deletion in three places which one place more of a phenylalanine(F) than other local reports. Comparing these sequences with Chinese PRRSV strains published after 2006,ORF5 gene shared 98.5%-99.7% of nucleotide homologies and 97.5%-99.5% of the deduced amino acid homologies, NSP2 gene shared 98.4%-99.3% of nucleotide homologies and 97.3%-98.9% of the deduced amino acid homologies. The phylogeneic tree revealed PRRSV-FJ09A strain isolated had closer relationship with PRRSV-FJ06A strain. According to the animal experiment PRRSV-FJ09A had a high pathogenicity to 30 d pigs. -
-
童光志,周艳君,郝晓芳,等.高致病性猪繁殖与呼吸综合征病毒的分离鉴定及其分子流行病学分析[J].中国预防兽医学报,2007,29(5):323-326. ZHOU L, ZHONG J L, ZENG J W, et al. The 30-Amino-Acid deletion in the Nsp2 of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China is not related to its virulence [J]. J Virol, 2009, 83(10):5156-5167.
-
期刊类型引用(6)
1. 林而舒. 不同解冻方式对半刺厚唇鱼风味物质的影响. 水产养殖. 2023(07): 1-5+17 . 
百度学术
2. 林而舒，胡振禧. 不同养殖密度对半刺厚唇鱼幼鱼生长特性的影响. 水产养殖. 2022(04): 34-37 . 百度学术
3. 蔡荔萱. 福建淡水土著鱼类养殖开发利用现状及发展建议. 福建畜牧兽医. 2021(04): 15-18 . 百度学术
4. 秦志清，林建斌，梁萍，章兆兴，邱曼丽，李学贵. 半刺厚唇鱼肌肉脂肪酸组成分析. 安徽农业科学. 2020(21): 192-194 . 百度学术
5. 秦志清，樊海平，林建斌，梁萍，叶小军，陈斌. 土池主养半刺厚唇鱼关键技术及效果研究. 福建农业科技. 2019(08): 46-48 . 百度学术
6. 梁萍，秦志清，林建斌，吴根飞，朱庆国，邱曼丽. 饲料中不同蛋白质水平对半刺厚唇鱼幼鱼生长性能及消化酶活性的影响. 中国农学通报. 2018(02): 136-140 . 百度学术
其他类型引用(0)
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
- 被引次数: 6
下载:
