水禽源禽1型副黏病毒强毒RT-PCR方法的建立

Development of RT-PCR for detecting virulent strains of waterfowl-origin avian paramyxovirus type 1

摘要: 参照GenBank上登录的水禽源禽1型副黏病毒强毒株F基因序列设计引物,建立检测水禽源禽1型副黏病毒强毒株的RT-PCR方法。该方法能从水禽源禽1型副黏病毒强毒株扩增出1条400 bp的特异片段,从水禽源禽1型副黏病毒弱毒、鸭瘟病毒、鸭1型肝炎病毒、减蛋综合征病毒、传染性法氏囊病病毒、H9亚型禽流感病毒和禽多杀性巴氏杆菌等均不能扩增出目的片段。敏感性试验显示该RT-PCR方法最低可检测出10 pg的病毒核酸。因此,该RT-PCR方法可用于水禽源禽1型副黏病毒强毒的临床诊断。

Abstract: An RT-PCR method was developed to detect virulent strains of waterfowl-origin avian paramyxovirus type 1(vwAPMV-1).The primers were designed according to the sequences of the fusion protein gene of vwAPMV-1s available at the GenBank.The result showed that the 400 bp of specific fragments were amplified in vwAPMV-1.However,negative results were obtained from Pasteurella multocide,EDS,DEV,IBDV,H9N2 influenza virus,DHV-1 and low virulent strains of waterfowl-origin avian paramyxovirus type 1.And,the 10pg of vwAPMV-1 RNA was amplified by the sensitivity test for the RT-PCR.The results indicated that the RT-PCR could be used for vwAPMV-1 detection.