氟苯尼考人工抗原的合成与鉴定

Synthesis and identification of florfenicol antigen

摘要: 采用混合酸酐(MA)法,将氟苯尼考(FFC)分别与牛血清白蛋白(BSA)和卵清蛋白(OVA)连接,制备人工免疫抗原FFC-HS-BSA和包被原FFC-HS-OVA,经紫外扫描(UV)、凝胶电泳(SDS-PAGE)、红外扫描光谱(IR)和动物免疫试验证实人工抗原合成成功。

Abstract: The immunogen,FFC-HS-BSA(florfenicol-bovine serum albumin),and the coating antigen FFC-HS-OVA(florfenicol-ovalbumin)were synthesized by using the mixed anhydride method.The man-made antigens were identified and certified by UV absorption,SDS-PAGE electrophoresis and animal immunization test.The results showed the process was successful.All antigens made had the correct haptens and carrier proteins.