摘要:
以文心兰茎尖为外植体,通过基本培养基(MS、1/2MS、1/2 N6)、植物激素(6-BA、NAA)、培养条件(有机添加物、糖、培养物状态)等关键因子对文心兰丛生芽诱导、增殖、生根各培养阶段影响的试验,探讨了文心兰以丛生芽途径再生植株的关键技术。结果表明:茎尖诱导丛生芽较适培养基配方为1/2 MS+6-BA2.0 mg•L-1+NAA 0.1 mg•L-1,诱导率为84.6%;丛生芽在MS+6-BA 2.0 mg•L-1+NAA 0.1 mg•L-1+蔗糖30 g•L-1培养基中增殖效果较好,增殖系数为5.5;株高2.0～3.0 cm的试管苗是丛生芽增殖的最佳培养材料;生根培养基适宜配方为1/2MS+IBA0.5 mg•L-1+苹果汁100 g•L-1+活性碳0.5 g•L-1+蔗糖20 g•L-1,生根率为100%。
关键词:
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文心兰
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丛生芽
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植株再生
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组织培养
Abstract:
Shoot tips of Oncidium were used as the explants to study the effect of some key factors on the induction,propagation and rooting of multiple shoots in Oncidium.The factors studied included the culture media(i.e.,MS,1/2MS and 1/2 N6),phytohormone(i.e.,6-BA and NAA) application and culture conditions(i.e.,organic additives,sugar and culture stages).From the results it was hoped that the core technology of Oncidium regeneration using its multiple shoots could be established.It was found that(a) the medium containing 1/2MS + 6-BA2.0mg•L-1 + NAA 0.1mg•L-1 was superior for the induction of multiple shoots from shoot tips,with a rate of 84.6%;(b) preferable propagation of multiple shoots was achieved on the medium containing MS + 6-BA 2.0mg•L-1 + NAA 0.1mg•L-1 + sugar 30g•L-1,with a 5.5 propagation coefficient;(c) test-tube plantlets of 2.0-3.0cm height were the optimal culture materials for the propagation;and(d) 100% rooting rate was obtained on the medium containing 1/2MS + IBA 0.5mg•L-1 + apple juice 100g•L-1+ activated carbon 0.5g•L-1 + sugar 20g•L-1.
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