优球蛋白法纯化欧鳗免疫球蛋白

基金项目:

国家863计划(2006AA100308);农业部科技专项(nyhyzx07-04315);福建省自然科学基金(2007FJ0057);福建省科技计划重大专项(2008N0024)

详细信息

Purification of Anguilla anguilla serum immunoglobulin by euglobulin precipitation

1.
Life Science Department of Fujian Normal University, Fuzhou, Fujian 350108, China;
2.
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

摘要: 采用优球蛋白法与饱和硫酸铵分步盐析法纯化欧洲鳗鲡血清免疫球蛋白(Ig),利用SDS-PAGE分析其纯度。试验结果显示:优球蛋白法纯化的欧鳗血清免疫球蛋白出现清晰2条带,纯度高于饱和硫酸铵分步纯化法;ELISA分析优球蛋白法纯化的欧鳗血清效价高于饱和硫酸铵纯化结果;Western-blot结果亦显示纯化的优球蛋白具有抗体免疫学活性。
- 免疫球蛋白 /
- 优球蛋白 /
- 纯化
Abstract: The purification of serum immunoglobulin from the European eel(Anguilla anguilla) was carried out by means of euglobulin as well as ammonium sulfate precipitation.Purified by euglobulin precipitation,the resultant material analyzed by SDS-PAGE showed two distinct protein bands,indicating a higher purity of the A.anguilla serum immunoglobulin than that obtained by ammonium sulfate precipitation.The ELISA analysis showed that the serum immunoglobulin purifid by euglobulin precipitation had a higher antibody activity than that purified by ammonium sulfate precipitation.Furthermore,the Western-blot antibody activity test also yielded a positive result on the serum immunoglobulin purified by euglobulin precipitation.
- imummoglobulin /
- euglobulin /
- purification