影响胡萝卜种子蛋白质提取的几种因素探讨

Factors affecting protein extraction from carrot seeds

摘要: 探讨了提取液比例、pH值、提取缓冲液、浸提时间、PVP用量、提取介质、SDS等因素对胡萝卜种子蛋白质提取的影响.结果表明:蛋白质的提取受料液比、pH值、浸提时间、缓冲液种类、提取介质的影响较大.初步确定,在4℃条件下,胡萝卜种子蛋白提取的较佳条件为:料液比(W:V)1:10,pH值8.0的50mmolL-1Tris-HCL缓冲液,浸提时间1 h.

Abstract: Factors including the ratio of sample to solution(W:V),pH value,buffers,time,amount of PVP,media and SDS that might affect the extraction of proteins from carrot seeds were studied.The results showed that the extraction was significantly affected by the ratio of sample to solution,pH,buffers,time and medium composition.Based on the preliminary results,protein extraction from carrot seeds for 1.0 h at 4℃ in 50 mmolL-1 Tris-HCl buffer with a 1:10 ratio of sample to solution and pH 8.0 produced the best result.